Optimization and Production of Amylase Enzyme from Crab Gut Microflora

Abstract

Crab receives bacteria in the gut from their aquatic environment through water and food that are populated with bacteria. Being rich in nutrients, the environment of the crab gut confers favourable conditions for the microorganisms. Enzymes are biological catalysts exceedingly esoteric with extraordinary catalytic power and embrace remarkable specificity. The objective of the present study was to improve the synthesis of amylase that was isolated from Bacillus sp., a type of crab intestine bacteria. In addition, many variables, including pH, temperature, and substrate concentration, were tuned. To generate higher and better enzyme activity, additional ions in the reaction media were categorised as activators or inhibitors. Based on this fact, crab waste can be effectively utilized for the production of amylases. The results illustrated in the table indicate that the optimum temperature for amylase activity was 33 ˚ C. The optimum temperature for amylase activity from MBK2 was similar to or quite higher than that of the optimum temperature of amylase from MBK1. The enzyme was highly active at pH 4.0 and it lost that optimum temperature for amylase activity was 33 ˚ C. The optimum temperature for amylase activity from MBK2 was similar to or quite higher than that of the optimum temperature of amylase from MBK1. The enzyme was highly active at pH 4.0 and it lost its activity considerably at pH 7. This pH optimum was higher than the pH optimum (11) of MBK1 and MBK2.